pETプラスミドのT7プロモーターの説明
T7 Promotor | T7 Promotor In pET Plasmid | pET Vector |
IPTG induction using the lac promoter
T7 RNA Polymerase & in vitro transcription (and more)
in vitro Transcription
IPTG-inducible protein expression using the lac promoter: just-the-gist version
プロモーターとエンハンサー
Tet on -Tet off system
発現システム
E.coli Expression Vectors: Cloned Gene Under Control of Regulated Promoter: λPL,T7, pBAD, pET vector
Protein Expression Vectors (pET vector) - Induction of Protein Expression (IPTG + T7 Pol) [Part 4]
Promoter Strength:lacUV5,hybrid promoters:tac,tic:factors affecting cloned gene expression in E.coli
Leveraging the Power of In Vitro Transcription
Expression vector, pBAD vector
RNA polymerase I structure
Phage pL promoter
Choosing vector plasmids and subcloning
RNA Polymerases
Optimization of Translational initiation sequences 5՚UTR for cloned gene in E.coli
Expression Vector | Cloning Vector |