Eukaryotic Transcription INITIATION - GTF assembly, CTD phosphorylation, Promoter escape/clearance

References/Resources: https://www.patreon.com/the_Crux

The first major step in the cycle of eukaryotic transcription - INITIATION! We talk about the assembly of general transcription factors (GTFs) associated with RNA polymerase II and look at the role of each GTF in the mechanistic production of a pre-initiation complex (PIC). The PIC formation is linked to the enhancer and we also see how they work together. After the formation of pre-initiation complex, the RNA polymerase II needs to clear the promoter region but when it does it pauses again - we discuss why that occurs. This video is a comprehensive advanced-dive into the initiation step of eukaryotic transcription.

Jump to your favorite section:
00:00 Outline
00:38 Step: PIC formation
01:38 TFIID
03:50 TFIIA
04:30 TFIIB
06:40 TFIIF & RNA pol
08:33 TFIIE & TFIIH
11:10 Step: Initiation
11:32 TFIIH & DNA scrunching
16:36 TFIIB & RNA pol blocking
18:07 Step: Promoter clearance
19:56 CTD phosphorylation
22:33 DSIF complex & NELF

Related videos:
Eukaryotic Transcription - The histone problem: https://youtu.be/KygzEjYfUts
Eukaryotic Transcription - Introduction: https://youtu.be/QmTf4M4qrbI
Eukaryotic Promoter Video: https://youtu.be/tZEWEQib_F8
Prokaryotic Transcription Initiation: https://youtu.be/JebsvaBCpQo

***Playlists that you must explore***
Advanced concepts in Transcription: https://www.youtube.com/playlist?list=PL0Ymnd-zt4Ij2VcAOHNUweftSElVsc-MX
Advanced concepts in Genetics: https://www.youtube.com/playlist?list=PL0Ymnd-zt4IgEBW8xWPZhHSN4O8HXqQ6j
Journal Club: https://www.youtube.com/playlist?list=PL0Ymnd-zt4IiRbmmqRbJdTskp4e5AhgrQ

Thanks for watching :)

Hit that LIKE button if you want more knowledge! COMMENT your questions, suggestions, and thoughts :) And I hope you have SUBSCRIBED because there is a lot more coming! :D